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in situ decellularization of tissues for high-resolution imaging

DATE
12 June 2016
PUBLICATION / RESOURCE TYPE
T1
TOPIC
  • Imaging
  • Proteomics
  • Metastasis

Summary

The extracellular matrix (ECM) is a master regulator of cellular phenotype and behavior. It has a crucial role in both normal tissue homeostasis and disease pathology. Here we present a fast and efficient approach to enhance the study of ECM composition and structure. Termed  in situ decellularization of tissues (ISDoT), it allows whole organs to be decellularized, leaving native ECM architecture intact. These three-dimensional decellularized tissues can be studied using high-resolution fluorescence and second harmonic imaging, and can be used for quantitative proteomic interrogation of the ECM. 

Source: http://www.nature.com/nm/journal/vaop/ncurrent/full/nm.4352.html 

Full text available here, with permission of fellow Sydney Catalyst member Thomas Cox, Garvan

 

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